Isolation and Characterization of a New Abortifacient Protein, Karasurin, from Root Tubers of Trichosanthes kirilowii MAX. var. japonicum KITAM.

Shunsuke TOYOKAWA; Tadahiro TAKEDA; Yukio OGIHARA

doi:10.1248/cpb.39.716

豊川峻輔, 竹田忠紘, 荻原幸夫

著者情報

キーワード: karasurin, Trichosanthes kirilowii var. japonicum, abortifacient protein, HPLC, amino acid sequencer, SDS-polyacrylamide gel electrophoresis

ジャーナルフリー

1991 年 39 巻 3 号 p. 716-719

DOI https://doi.org/10.1248/cpb.39.716

詳細

抄録

A new abortifacient protein, named karasurin, was isolated from fresh root tubers of Trichosanthes kirilowii MAXIMOWICZ var. japonicum KITAMURA (Cucurbitaceae, Japanese name : kikarasuuri) by the procedure involving acetone fractionation and ion-exchange chromatography on Toyopearlpak SP 650S. Homogeneity of Karasurin was demonstrated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, and high performance liquid chromatography (HPLC). Karasurin was a highly basic protein of pI 10.1 and the molecular weight was estimated as 28000 by SDS-polyacrylamide gel electrophoresis. Karasurin showed a strong abortion effect in pregnant mice.

責任著者(Corresponding author)

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