Abstract
A reversed-phase high-performance liquid chromatographic method was developed for the determination of sulfur in topical pharmaceutical preparations. The mobile phase consisted of methanol-5% acetic acid water (85 : 15). The analysis was carried out using an octadecyl silica column (5 μm, 4.0 mn i.d.×125 mm), with spectrophotometrical detection at 254 nm. Pyrene was used as an internal standard. The calibration curve was linear with a correlation coefficient >0.9996 in the range of 1-3 μg sulfur injected. This method is very useful in determination of sulfur in ointments containing other antibacterial or antiinflammatory drugs.
