Pyranine Phosphate as a New Fluorogenic Substrate for Acidic and Alkaline Phosphatases

Eisuke SATO; Kazuyoshi CHIBA; Motonori HOSHI; Yuichi KANAOKA

doi:10.1248/cpb.40.786

Eisuke SATO, Kazuyoshi CHIBA, Motonori HOSHI, Yuichi KANAOKA

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Keywords: pyranine, quenching, fluorogenic substrate, acid phosphatase, alkaline phosphatase, fluorometric enzyme assay

JOURNAL FREE ACCESS

1992 Volume 40 Issue 3 Pages 786-788

DOI https://doi.org/10.1248/cpb.40.786

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Abstract

8-Hydroxypyrene-1, 3, 6-trisulfonic acid (HPTS, pyranine) is highly fluorescent not only in the alkaline range but also at an acidic pH, and in a water-soluble fluorescent compound. By phosphorylation of a pyranine, its fluorescence intensity was quenched markedly. A pyranina phosphate was shown to be a potential fluorogenic substrate for the assay of acid and alkaline phosphatases, and also for phosphatase activities in human serum.

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