Abstract
Four novel maltopentaosides, 20-chloro-4-nitorphenyl O-(6-O-p-toluenesulfonyl α-D-glucopyranosyl)-(1→4)-tris[O-α-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside (4), 2-chloro-4-nitrophenyl O-[6-O-(tert-butyldimethyl)silyl-α-D-glucopyranosyl]-(1→4)-tris[O-α-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside (5), 2-chloro-4-nitropheny O-[6-deoxy-6-(phenyl)sulfonyl-α-D-glucopyranosyl]-(1→4)-tris[O-α-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside (10), and 2-chloro-4-nitorphenyl O-(6-deoxy-6-phthalimido-α-D-glucopyranosyl)-(1→4)-tris[O-α-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside (11) were synthesized. Substrates 4, 5, 10, and 11 were hydrolyzed by human pancreatic α-amylase (HPA) from 1.1 to 2.9-fold faster than by human salivary α-amylase (HSA). Taking adventage of the difference in the hydrolytic rate of 5(2.9-fold faster), we developed a new method for the differential assay of these two human α-anylases.
