Screening of Tissue Cultures and Thalli of Lichens and Some of Their Active Constituents for Inhibition of Tumor Promoter-Induced Epstein-Barr Virus Activation

Yoshikazu YAMAMOTO; Yasutaka MIURA; Yasuhiro KINOSHITA; Masako HIGUCHI; Yasuyuki YAMADA; Akira MURAKAMI; Hajime OHIGASHI; Koichi KOSHIMIZU

doi:10.1248/cpb.43.1388

Yoshikazu YAMAMOTO, Yasutaka MIURA, Yasuhiro KINOSHITA, Masako HIGUCHI, Yasuyuki YAMADA, Akira MURAKAMI, Hajime OHIGASHI, Koichi KOSHIMIZU

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Yoshikazu YAMAMOTO
Yasutaka MIURA
Yasuhiro KINOSHITA
Masako HIGUCHI
Yasuyuki YAMADA
Akira MURAKAMI
Hajime OHIGASHI
Koichi KOSHIMIZU
Author's Organization：
Central Research Institute, Nippon Paint Co., Ltd.,
Central Research Institute, Nippon Paint Co., Ltd.,
Central Research Institute, Nippon Paint Co., Ltd.,
Central Research Institute, Nippon Paint Co., Ltd.,
Graduate School of Science and Technology, Nara Institute of Science and Technology
Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University
Food Science and Technology, Faculty of Agriculture, Kyoto University
Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University

Keywords: Epstein-Barr virus activation inhibitor, lichen tissue culture, lichen secondary metabolite

JOURNAL FREE ACCESS

1995 Volume 43 Issue 8 Pages 1388-1390

DOI https://doi.org/10.1248/cpb.43.1388

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Abstract

Inhibition of tumor promoter-induced Epstein-Barr virus (EBV) activation was screened using tissue culture and thallus extracts of lichens. Usnea longissima ACH. thallus and Cetraria ornata MULL. ARG. tissue culture showed strong inhibitory activity. We identified (+)-usnic acid (1), barbatic acid (2), diffractaic acid (3), 4-O-demethylbarbatic acid (4), and evernic acid (5) as inhibitors of EBV activation from the U. longissima thallus. Of these compounds, (+)-usnic acid exhibited the highest inhibitory activity (IC₅₀=1.0 μM).

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