Characterization and Determination of Human Urinary Keratan Sulfate

Hidenao TOYODA; Yoshikuni DEMACHI; Shizuyo KOMORIYA; Nobuyoshi FURUYA; Toshihiko TOIDA; Toshio IMANARI

doi:10.1248/cpb.46.97

Hidenao TOYODA, Yoshikuni DEMACHI, Shizuyo KOMORIYA, Nobuyoshi FURUYA, Toshihiko TOIDA, Toshio IMANARI

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Keywords: human urine, keratan sulfate, keratanase, HPLC, ^1H-NMR

JOURNAL FREE ACCESS

1998 Volume 46 Issue 1 Pages 97-101

DOI https://doi.org/10.1248/cpb.46.97

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Abstract

Keratan sulfate was isolated from normal human urine was characterized by sugar compositional analysis and ¹H-NMR spectroscopy. It was found that KS from human urine is classified as skeletal type (KS-II type chain) with an O-glycosidic linkage between galatosamine and serine (or theronine). ¹H-NMR studies revealed that urinary KS is not a proteoglycan but a polysaccharide (molecular weight is about 5kDa). The quantitation of human urinary KS by HPLC showed that urinary KS is excreted at constant levels (0.07±0.015 μg/mg creatinine).

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