Abstract
A newly prepared copper(II)-complexed β-cyclodextrin plymer cross-linked with epichorohydrin was used as a packing material with a strongly alkaline eluate for the liquid chromatography of nucleosides. Nucleosides such as adenosine and cytidine were retained on the column because of their strong interaction with copper(II) ions. On the other hand, 2'-deoxydanosine was retained on the copper(II)-free and copper(II)-complexed columns because of the inclusion property of β-cyclodextrin. These findings indicate that chromatography separations can be achieved by the inclusion effect and copper(II)-complexation.
