Determination of Secnidazole in Urine by Adsorptive Stripping Voltammetry

Abstract

Cyclic voltammetry was used to explore the adsorption behavior of secnidazole on a hanging mercury drop electrode (HMDE). The effects of various operational parameters on the accumulation behavior of the adsorbed species were tested. Thus, a sensitive stripping voltammetry procedure for the determination of secnidazole with an adsorptive accumulation on the surface of HMDE has been developed. Measurements were taken by differential-pulse voltammetry after determination of the optimum conditions. The linear concetration range was 1×10^-8-1×10^-7M when using a 120 s preconcentration at -0.1 V vs. Ag/AgCl in acetate buffer of pH 4.0. The detection limit of secnidazole was 5×10^-9M. The precision, expressed by the coefficient of variation, was 2.5% (n=10) at a concentration of 1×10^-7M. The method was successfully applied to the analysis of secnidazole in urine.