Abstract
The urinary metabolites of p-dimethylaminoazobenzene (DAB) in dog were studied, and in this connection, the metabolites of p-aminoazobenzene (AB) and 4'-hydroxy-4-aminoazobenzene (4'-OH-AB) were also examined. From the hydrolyzed 24-hr. urine after administration of DAB, o-aminophenol, p-aminophenol, aniline, p-phenylenediamine, AB, and p-methylaminoazobenzene (MAB) were detected. The amount of o-aminophenol was larger than that of the other amines. By paper chromatography 5-phenylazo-2-aminophenyl hydrogen sulfate (3-HSO3O-AB), 3-HSO3O-MAB, and 4'-HSO3O-AB were respectively identified from the 3- to 7-hr. DAB-urine. The presence of 3-HSO3O-AB N-glucosiduronic acid in the DAB- and AB-urine was assumed from their paper chromatographic behavior.
