Abstract
According to the method reported previously 4-ribitylaminouracil was produced from 4-chlorouracil and D-ribamine, and its nitroso derivative was reduced to 4-ribitylamino-5-aminouracil, which was isolated as hydrochloride or sulfite. Reaction of the salt with pyruvic acid in the presence of hydrazine afforded 6-methyl-7-hydroxy-8-D-ribityl-2 (3H), 4 (8H)-pteridinedione, m.p. 263°(decomp.), which was in complete accord in every property with purple fluorescent 6-methyl-7-hydroxyribolumazine isolated from the mycelium of Eremothecium ashbyii.
