Abstract
To find the minimum structural requirement in the substrate for pancreatic ribonuclease (RNase-I-A), 5-O-benzyl-D-ribofuranoside 2, 3-cyclic phosphate (VI), and methyl α- and β-D-ribofuranoside 2, 3-cyclic phosphates (IX and X) were synthesized and submitted to hydrolysis with this enzyme. No evidence was observed for hydrolysis of these cyclic phosphates to the corresponding 3-phosphates by the RNase and the results showed that more specially arranged atom grouping is required at 1-position of D-ribofuranoside 2, 3-cyclic phosphate for this enzymatic hydrolysis.
