Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Application of Chromatography. XLIII. On the Mechanism of Enzymic Conversion of 6, 7-Dimethylribolumazine to Riboflavin.
Mitsuko AsaiToru MasudaSatoru Kuwada
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1961 Volume 9 Issue 6 Pages 503-509

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Abstract
It was previously found that reaction between 6, 7-dimethylribolumazine and a crude enzyme prepared from the mycelium of Er. ashbyii produced riboflavin and 6-methyl-7-hydroxyribolumazine and reported that the enzymic reaction yielded a considerable amount of riboflavin without addition of any carbon donor. Later, the products in the enzymic reaction were investigated by paper partition chromatography and a bluish green fluorescent spot with Rf 0.10 was detected besides the known substances, and the newly found substance was assumed to be the dimeride of 4-ribitylamino-5-aminouracil described in part XLI of this series.
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© The Pharmaceutical Society of Japan
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