抄録
Procedures of determining parameters of pre-freezing process are examined experimentally as well as analytically. The critical cell volume ratio of the endothelial cell and the smooth muscle cell of porcine arteries, which are closely related to the critical osmotic pressure, are measured from the view point of physiological functions. Also, the membrane permeability of endothelial cell is measured. By comparing two pre-freezing processes, that is, the direct immersion to the glycerol solution and the process in which the concentration of glycerol solution is increased stepwise, validity of the designing method is assessed experimentally.
