低温生物工学会誌
Online ISSN : 2424-1555
Print ISSN : 1340-7902
19. シロイヌナズナ培養細胞の乾燥および超低温耐性(平成14年度第48回低温生物工学会研究報告)
西 加奈子菅原 康剛
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2002 年 48 巻 2 号 p. 157-160

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Suspension-cultured cells of Arabidopsis thaliana were precultured in media with high concentrations of sucrose or sorbitol and their tolerance to desiccation and super-low temperature was examined. The tolerance of the cell to desiccation and super-low temperature substantially increased after preculturing in sucrose- or sorbitol-enriched medium. Osmotic concentration of cell-free extract increased and considerable amounts of sucrose and proline accumulated in the cell during precuturing in sucrose- or sorbitol-enriched medium. It was suggested that the accumulation of these substances play an important role in the development of the tolerance in A. thaliana cells.
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© 2002 低温生物工学会
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