抄録
Suspension cells of Daucus carota precultured in MS medium containing 0.4 M sucrose for 5 days showed enhanced desiccation tolerance and could be preserved in liquid nitrogen with a desiccation method. Protoplasts were isolated from precultured carrot cells by treatment with Driselase and then cultured. Although cell division rate was lower than that of the protoplasts isolated from non-precultured control cells, cell division of protoplast isolated from precultured cells was observed. Protoplasts isolated from the precultured cells were desiccated and rehydrated. Viability of rehydrated protoplasts was determined after staining with Evans blue. Some protoplasts that were not stained seemed to be alive.
