Cell Structure and Function
REVIEW
Live Cell Imaging Using Wide-Field Microscopy and Deconvolution
Jason R. SwedlowMelpomeni Platani
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Volume 27 (2002) Issue 5 Pages 335-341

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Abstract

The use of fluorescence imaging methods, most recently based on fluorescent protein technology, and the availability of high quality fluorescence imaging systems have driven a revolution in cell and molecular biology. Live cell imaging, especially using fluorescence, is now used in a wide variety of assays in academic and commercial laboratories. The use of this technology requires particular attention to be paid to cell engineering, the design of the image acquisition system, the imaging protocol, and subsequent processing and analytic methods. In this review, we discuss each of these steps, highlighting practical techniques developed by us and others.

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© 2002 by Japan Society for Cell Biology
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