Novel Method for Substance Injection into the Cell by Laser Beam

A Study of the Injection Volume

Abstract

A DNA transfection method by laser microbeam pricking has been recently reported (Kurata, S. et al. Exp. Cell Res. 162, 372 (1986)). The volume of external fluid transferred into the cell by the method was determined through the injection of diphtheria toxin fragment A (Yamaizumi, M. et al. Cell 15, 245 (1978)). Using these results and the results on laser DNA transfection efficiency (Kurata, S. et al. Exp. Cell Res. 162, 372 (1986)), the approximate number of DNA molecules necessary to transform the recipient cell was estimated.