Abstract
Canine carotid arterial and external jugular venous endothelial cells were derived, cutured, passed, and pooled. Three × 106 cells were seeded into capped roller-bottles. Three roller-bottes were seeded with venous, and another three with arterial derived endothelial cells for each of three "oxygen saturations" 5, 20 and 50 % O2 respectively, and incubated for seven days and then counted. Arterial endothelial cells showed no cell proliferation in high oxygen saturation compared to an 83 % increase in cell numbers when oxygen supply had been held to 20 %. Venous endotheial cells showed no cell pro-liferation in medium or high oxygen supply but low oxygen supply (5 % O2) gave a 91 % increase in cell counts. The small number of roller-bottles investi-gated does not allow firm conclusions but our results warrant further evalu-ation.
