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Volume 12 (1987)
- Issue 6 Pages 509-
- Issue 5 Pages 407-
- Issue 4 Pages 317-
- Issue 3 Pages 225-
- Issue 2 Pages 141-
- Issue 1 Pages 1-
Volume 12, Issue 2
Displaying 1-9 of 9 articles from this issue
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Sei-ichi Ishiguro, Kaoru Fukuda, Chin-ichiro Kanno, Katsuyoshi Mizuno1987 Volume 12 Issue 2 Pages 141-155
Published: 1987
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSImmunoreactive opsin was detectable in the apical portion of normally developing photoreceptor cells on postnatal day 3 by the indirect enzyme-labeled antibody method. Immunoreactivity increased and had ex-tended from the central retina to the periphery by the advanced stages of development. In the rd mutant retinas, accumulated opsin was present in the apical portion and in the outer nuclear layer on postnatal day 8. Immuno-reactive opsin mainly was present in the outer nuclear layer by day 14, even being detectable on day 28. No immunoreactivity was present in the remaining cones. Electron microscopic immunocytochemistry confirmed the association of immunoreactive opsin with the persistent rod cell plasma membrane. Mole-cular weight of immunoreactive opsin in 14-day-old rd mutant mouse retina, as estimated by gel filtration chromatography, was large and did not seem to be degraded. These findings indicate that accumulated rhodopsin continues to function in the plasma membrane because an electroretinogram could be made after day 14 for the rd mutant mouse retina.View full abstractDownload PDF (11298K) -
Kazuhide Takahashi, Ichiro Kaneko, Keiji Fushimi1987 Volume 12 Issue 2 Pages 157-163
Published: 1987
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSExtensive digestion of Chinese hamster metaphase chromo-somes with Alu I, Hae III and Hinf I released up to 40 distinct chromosomal proteins. Some of the proteins released by Hae III or Hinf I were enriched in the protein moiety liberated by Alu I but several proteins released by Hae III were not released by Alu I digestion. The amount of chromosomal protein released by deoxyribonuclease I (DNase I) was comparable to that liberated by the three restriction enzymes so far tested, while only four abundant protein species were detectable in the protein moiety released by DNase I. Two of them with molecular weights of 58, 000 and 50, 000 were also released by the three restriction enzymes and are similar in size to those found previously in the core-like structure of histone-depleted chromosomes.View full abstractDownload PDF (3654K) -
Hiroaki Asou, Shusuke Hirano1987 Volume 12 Issue 2 Pages 165-171
Published: 1987
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSThe ganglioside concentration and composition in growth cone-deficient nerve cells, induced by inclusion of cytochalasin B (CB) are compared with those of 2-day-old control cells from primary cultures of embryonic rat cerebral cortex. Ganglioside GM1 and GD1a are the major gangliosides in the growth cone. Ganglioside GM1 may be one of the membrane components of growth cones that function in neural recognition during development.View full abstractDownload PDF (558K) -
Hiroko Hama-Inaba, Mitsuko Takahashi, Michiki Kasai, Tadahiro Shiomi, ...1987 Volume 12 Issue 2 Pages 173-180
Published: 1987
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSA pulse-generating machine which delivers exponentially decaying pulses over broad range of pulse lengths was used to determine the optimum pulse conditions for gene transfer to FM3A cells. In the transformation of tk- cells with pTK1, a single pulse of 100-2000 μs gave a high transformation frequency at 1.5-6 kV/cm and room temperature, the highest transformation frequency obtained being 3 x 10-3. As the suspension buffer for cells exposed to the pulse, Saline G was better than PBS(-)for obtaining a large number of transformants because it ensured high cell viability.View full abstractDownload PDF (804K) -
Masanori Murakoshi, Yoshiyuki Osamura, Keiichi Watanabe1987 Volume 12 Issue 2 Pages 181-186
Published: 1987
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSLocalization of glutathione-peroxidase (GSH-PO) in adrenal cortical cells of neonatal rats were determined by immunocytochemical analysis. GSH-PO first appeared 14 days after birth. Intracellular localization of GSH-PO was mainly in cytosol (cytosol GSH-PO) but no intramitochondrial localization of GSH-PO was detected. Twenty-one days after birth, intramitochondrial localization of GSH-PO (mitochondrial GSH-PO) was present. Mitochondrial GSH-PO depends on ACTH stimulation; therefore, its presence in the adrenal cortical cells of neonatal rats may be the morphologic expression of the gradual acquisition of adult metabolic features during steroidogenesis. The intracellular GSH-PO staining pattern in adrenal cortical cells therefore should be a useful marker for steroidogenesis.View full abstractDownload PDF (5678K) -
Eiji Okimasu, Koichi Nobori, Sumio Kobayashi, Etsuko Suzaki, Shigeo Te ...1987 Volume 12 Issue 2 Pages 187-195
Published: 1987
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSAlthough cholesterol is one of the major components of plasma membranes in eukaryotic cells, very little is known about its role in biological membranes. We reported previously (Okimasu et al., Cell Struct. Funct. 11, 273-283, 1986) that introduction of cholesterol into the liposomal membrane caused a decrease in membrane permeability, especially by the binding of cytoplasmic proteins to the liposomal membrane. The present study was carried out to further clarify the biochemical function of cholesterol in the membrane-protein interactions, especially under high osmotic pressure.
The association of membranes with cytoplasmic proteins and their permeability were decreased by the introduction of cholesterol, but its effects were diminished in a hypertonic medium. The protein species associated with cholesterol-containing liposomes vary depending on the sort of hypertonic condition. It was suggested that since the degree of lipid packing by the cholesterol was reduced by the locally increased curvature in the lipid bilayer under high osmotic pressure, some cytoplasmic proteins can penetrate into the liposomal membrane.View full abstractDownload PDF (815K) -
Toshio Ohyama, Michio Ito1987 Volume 12 Issue 2 Pages 197-203
Published: 1987
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSNuclei of lily microsporocytes in meiotic prophase were sub-j ected to two-dimensional spreading and stained with silver nitrate. This method made it possible to observe the behavior of synaptonemal complexes with light microscopy. Axial cores of homologous chromosomes, which had been formed at the leptotene stage, were paired during the zygotene stage, consequently being integrated into the synaptonemal complexes. During the middle zygotene stage, a number of paired segments were observed as heavily stained stretches in the spread nuclei, thus indicating the multiple initiation of pairings along sets of homologous chromosomes.View full abstractDownload PDF (5422K) -
Reiko Sasada, Haruo Onda, Koichi Igarashi1987 Volume 12 Issue 2 Pages 205-217
Published: 1987
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSExpression plasmids containing human interleukin-2(IL-2) cDNA under the control of viral promoters (SV40 early region, MuLV LTR, HTLV-I LTR, and ASV(Y73) LTR) were introduced into TK- mouse L cells and human FL cells to establish IL-2 producing cells. The highest levels of IL-2 producing clones were obtained in TK+ mouse L cells transformed with a recombinant plasmid having MuLV LTR as a promoter, whereas trans-formed cells of human FL cells (G418r) were revealed to produce IL-2 at the highest level when the cells were transfected with a plasmid containing HTLV LTR as a promoter. These results suggest that these promoter/enhancer regions possess different cell specificities in gene expression. To obtain higher levels of IL-2 production using gene amplification, the hybrid plasmids containing the hamster DHFR and human IL-2 genes were constructed and transfected into DHFR-CHO cells. DHFR+ colonies produced IL-2 at about the same level as that produced by TK+ L cells transformed with the recombinants containing MuLV LTR. Selection of methotrexate-resistant cells resulted in a 5-to 30-fold increase of IL-2 production. These cells produced IL-2 stably for at least 3 months, even in the absence of methotrexate.View full abstractDownload PDF (4490K) -
Bengt Lindblad, William E. Burkel, James C. Stanley1987 Volume 12 Issue 2 Pages 219-221
Published: 1987
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSCanine carotid arterial and external jugular venous endothelial cells were derived, cutured, passed, and pooled. Three × 106 cells were seeded into capped roller-bottles. Three roller-bottes were seeded with venous, and another three with arterial derived endothelial cells for each of three "oxygen saturations" 5, 20 and 50 % O2 respectively, and incubated for seven days and then counted. Arterial endothelial cells showed no cell proliferation in high oxygen saturation compared to an 83 % increase in cell numbers when oxygen supply had been held to 20 %. Venous endotheial cells showed no cell pro-liferation in medium or high oxygen supply but low oxygen supply (5 % O2) gave a 91 % increase in cell counts. The small number of roller-bottles investi-gated does not allow firm conclusions but our results warrant further evalu-ation.View full abstractDownload PDF (284K)
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