Kinetics of the Clonal Proliferation of Granulocytes and Macrophages in Cultures of Mouse Bone Marrow Cells as Supported by Two Distinct Types of Colony-Stimulating Factors

抄録

Two different types of colony-stimulating factors (CSF) were used to support the clonal growth of myeloid progenitor cells (CFU_C) in semi-solid agar or viscous methylcellulose cultures of mouse bone marrow cells. The cultures stimulated for 5 days with RSP-2•P3 cell CSF (CSF_RSP) contained mainly granulocyte colonies, whereas the cultures stimulated for 10 days with human urine CSF (CSF_hu) contained mainly monocyte/macro-phage colonies. Four lines of study were carried out : 1) a kinetic study using combinations of the two types of CSF_S in the same culture; 2) a study of transferring CFU_C from the initial 3-day cultures to recipient cultures con-taining the same or different types of CSF; 3) an examination of the morpholo-gy over time of colonies that were confined by glass capillaries plunged in agar; and 4) electron microscopic observations on disintegrating granulocytes. The results of all these lines of study suggest that about one third of the CFU_C can be stimulated both by CSF_RSP and CSF_hu while the other two thirds react specifically either with CSF_RSP or with CSF_hu. The present study also suggests that granulocytes in the culture stop proliferation and disintegrate while macrophages are still growing there. Thus, mixed-type colonies con-taining both macrophages and granulocytes later become macrophage colonies.