Abstract
A simple method is described for in situ freeze-cleaving of fibroblasts grown on the Falcon plastic culture dish. The culture plate with cells is covered by another Falcon plastic plate previously coated with rat tail collagen so to sandwich the cells. The culture plate is then set on the freeze-etching apparatus. The freeze-etching apparatus used was the cold metal block type principally based on Bullivant and Ames' method. Fracturing was per-formed by removing the cover or the culture plastic plates. By setting the cul-ture plate below or above on the specimen stage, this method selectively exposed the EF and PF fracture faces of both the free surface and substrate contact surface of cultured cells.
