Abstract
Cultured muscle cells treated successively with saponin at varied concentrations released protein initially at 2 x 10-5 g/ml saponin and then at 6 x 10-5 g/ml saponin. The first release was accompanied with creatine kinase activity, whereas the second release was not. Morphological observations indicated that the first protein leakage was derived mainly from myotubes and the second release mainly from mononucleated cells.
