Quantitative Cryoimmunogold Electron Microscopic Studies on Induction of Serine : pyruvate Aminotransferase in Rat Liver Mitochondria by Administration of Glucagon

Shingo Satoh; Akitsugu Yamamoto; Koji Iwata; Toshiaki Oda; Kyu-Ichiro Okuda; Syushi Higashi; Toshiaki Setoguchi; Yutaka Tashiro

doi:10.1247/csf.20.89

Shingo Satoh, Akitsugu Yamamoto, Koji Iwata, Toshiaki Oda, Kyu-Ichiro Okuda, Syushi Higashi, Toshiaki Setoguchi, Yutaka Tashiro

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Keywords: quantitative immunogold electron microscopy, cryoultrathin section, serine : pyruvate aminotransferase, rat liver, mitochondria

JOURNAL FREE ACCESS

1995 Volume 20 Issue 1 Pages 89-96

DOI https://doi.org/10.1247/csf.20.89

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Abstract

Induction of mitochondrial serine : pyruvate aminotransferase (SPT) in rat liver by administration of glucagon was studied quantitatively by immunoblot analysis and cryoimmunogold electron microscopy.Immunoblot analysis revealed that two daily injections of glucagon produced marked increase of SPT protein mass to a level as much as 18 times that of the untreated rat. Cryoimmunogold electron microscopic analysis showed that the labeling density of the mitochondria increased in a parallel manner. Thus the induction of SPT analyzed by two methods showed an excellent correlation with a relative correlation coefficient of 0.98, indicatingthat the induction of SPT can be analyzed quantitatively by immunogold electron microscopy on cryoultrathin sections.

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