Abstract
The inhibitory effects of aphidicolin on growth and DNA synthesis in vivo in mouse FM3A cells were reversed by the simultaneous addition of all four deoxyribonucleosides to the culture. In lysolecithin-permeabilized FM3A cells, the inhibition of DNA synthesis by aphidicolin also was reversed by increasing the concentrations of all four dNTP's, but not by increasing the concentrations of three of the four. DNA synthesis in per-meabilized cells exhibited a close resemblance to that in vivo with respect to its sensitivity to aphidicolin and its type of inhibition. In contrast, a 100-times higher concentration of aphidicolin was required to inhibit DNA polymerase a and its inhibition was reversed only by dCTP.
