Abstract
Ethidium bromide has been shown to be useful as a probe to observe mitochondrial nuclei (mt-nuclei) in the moving mitochondria in live cells. In Physarum polycephalum, a double staining technique, using ethidium bromide and dimethylaminostyrylmethylpyridiniumiodine, detects both changes in mitochondrial distribution and orientation, and changes in the shape of the mt-nucleus during the mitochondrial division cycle. The results show that when dumbbell-shaped mitochondria in live cells divide to form daughter mito-chondria, each mt-nucleus also divides, separating into the daughter mito-chondria.
