抄録
We investigated the selective uptake of liposomes chemically modified by polysaccharides-cholesterol derivatives (CHP) with 1-amino-lactose (lactose CHP) in a rat hepatoma cell line (AH66), two human hepatoma cell lines (HuH7 and Alexander), a human colon cancer cell line (FCC) and a human lung cancer cell line (KNS), respectively. Uptake of 3H-labeled lactose CHP-coated liposomes was 2.9 times greater than that of CHP-coated liposomes in the AH66, 4.4 times in the HuH7, 4.7 times in the Alexander, 3, 4 times in the FCC and 4.4 times in the KNS after 3-hours of incubation. In order to clarify the mechanism of this phenomenon, we also investigated the uptake of two polymers with aminolactose or lactonamide in the AH66, the Alexander and the FCC. A human hepatoma cell line (HepG2) which is proved to have the galactose receptor was also studied. One polymer was poly(vinylbenzylaminolactose-co-vinylbenzylaminomaltose) which has a 90% induction rate of aminolactose and another was poly(vinylenzyllactonamide-co-vinylbenzylmaltonamide) which has a 90% induction rate of lactonamide. The uptake of poly(vinylbenzylaminolactose-co-vinylbenzylaminomaltose) was greater than that of poly(vinylbenzyllactonamide-co-vinylbenzylmaltonamide) in the AH66, the Alexander and the FCC, although the uptakes of them were the same in the HepG2. But, adversely the uptake of poly(vinylbenzyllactonamide-co-vinylbenzylmaltonamide) was greater than that of poly(vinylbenzylaminolactose-co-vinylbenzylaminomaltose) in hepatocytes. Uptake ratios of the two polymers into hepatocytes were higher than those of cancer cells. Our results suggest that aminolactose has a higher affinity with cancer cells than lactonamide and that lactose possibly has the higher affinities with cancer cells than galactose except for the HepG2.
