抄録
In the present study, we investigated the resistance of plasmid DNA, which is formed a complex with cationic liposomes, to nuclease using pGreen Lantern plasmid coding green fluorescent protein of Aquorea victoria jellyfish. Native plasmid DNA was digested easily with above 10% of fetal bovine serum and 1 × 10-4 units of DNase I from bovine pancreas by the estimation of agarose-gel electrophoresis. On the other hand, plasmid DNA complexed with cationic liposomes composed of TMAG exhibited the resistance to DNase, and relaxed and supercoiled plasmid DNA were observed on the same electrophoretic mobility at the same intensity as control plasmid. The degradation of plasmid DNA by DNase was estimated quantitatively with NIH image plot profile analysis, and the recovery of plasmid DNA showed the almost same values irrespective of DNase treatment. These findings suggest that plasmid DNA acquires the resistance to DNase on account of the complex formation with cationic liposomes.
