Apoptotic and Necrotic Influence of Dental Resin Polymerization Initiators in Human Gingival Fibroblast Cultures

Abstract

The aim of this study was to examine the apoptotic and necrotic influence of four dental resin polymerization initiators—namely benzoyl peroxide (BPO), camphorquinone (CQ), dimethylaminoethyl methacrylate (DMAEMA), and dimethyl-para-toluidine (DMPT)—on human gingival fibroblast (HGF) cells. To this end, the growth inhibition of HGF cells with 1 mM BPO, CQ, and DMAEMA, and 500 μM DMPT was evaluated using Cell Counting Kit-8. Then, cell cycle analysis by flow cytometry was used to assess propidium iodide-stained cells (distribution of cells in G₀/G₁, S, G₂/M phases). All four dental resin polymerization initiators induced G₀/G₁ cell cycle arrest. As for the patterns of cell death (necrosis and/or apoptosis), they were analyzed using Annexin V-FITC/PI staining with flow cytometry. All four dental resin polymerization initiators most likely induced necrosis.