3-dimensional composite scaffolds consisting of apatite-PLGA-atelocollagen for bone tissue engineering

Masaaki TAKECHI; Koji OHTA; Yoshiaki NINOMIYA; Misato TADA; Masayuki MINAMI; Megumi TAKAMOTO; Akira OHTA; Takayuki NAKAGAWA; Akiko FUKUI; Youji MIYAMOTO; Nobuyuki KAMATA

doi:10.4012/dmj.2011-182

Original Paper

3-dimensional composite scaffolds consisting of apatite-PLGA-atelocollagen for bone tissue engineering

Masaaki TAKECHI, Koji OHTA, Yoshiaki NINOMIYA, Misato TADA, Masayuki MINAMI, Megumi TAKAMOTO, Akira OHTA, Takayuki NAKAGAWA, Akiko FUKUI, Youji MIYAMOTO, Nobuyuki KAMATA

Author information

Masaaki TAKECHI
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
Koji OHTA
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
Yoshiaki NINOMIYA
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
Misato TADA
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
Masayuki MINAMI
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
Megumi TAKAMOTO
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
Akira OHTA
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
Takayuki NAKAGAWA
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
Akiko FUKUI
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
Youji MIYAMOTO
Department of Oral Surgery, Subdivision of Molecular Oral Medicine, Division of Integrated Sciences of Translational Research, Institute of Health Biosciences, The University of Tokushima Graduate School
Nobuyuki KAMATA
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University

Keywords: PLGA, Apatite, Atelocollagen, Scaffold, Bone tissue engineering

JOURNAL FREE ACCESS

2012 Volume 31 Issue 3 Pages 465-471

DOI https://doi.org/10.4012/dmj.2011-182

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Abstract

We fabricated 3-dimensional scaffolds consisting of biodegradable poly(D, L-lactide-co-glycolic acid)(PLGA)(75/25) with hydroxyapatite particles containing atelocollagen (aAC). The aim of this study was to evaluate this new type of scaffold in regard to its basic properties and biocompatibility. Characterization of the obtained scaffolds was performed to know the porosity, shrinkage, diametral tensile strength, and biocompatibility. Composite scaffolds made of PLGA with hydroxyapatite particles containing atelocollagen (PL-aAC) showed a greater strength and stability than PLGA scaffolds. PL-aAC also exhibited superior performance in terms of cell attachment and proliferation as compared to PLGA, while histological findings showed that PL-aAC had an excellent response toward soft tissues. Our results strongly suggest that PL-aAC is more useful for cell transplantation as compared to PLGA for bone tissue engineering.

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