Dental Materials Journal
Online ISSN : 1881-1361
Print ISSN : 0287-4547
ISSN-L : 0287-4547
Original Paper
Effects of fish collagen peptides on collagen post-translational modifications and mineralization in an osteoblastic cell culture system
Shizuka YAMADAHideaki NAGAOKAMasahiko TERAJIMANobuaki TSUDAYoshihiko HAYASHIMitsuo YAMAUCHI
Author information
JOURNALS FREE ACCESS

2013 Volume 32 Issue 1 Pages 88-95

Details
Abstract

Collagen is one of the most widely used biomaterials for tissue engineering and regenerative medicine. Fish collagen peptides (FCP) have been used as a dietary supplement, but their effects on the cellular function are still poorly understood. The objective of this study was to investigate the effects of FCP on collagen synthesis, quality and mineralization using an osteoblastic MC3T3-E1 cell culture system. Cells treated with FCP significantly upregulated the gene expression of several collagen modifying enzymes and more collagen was deposited in the cultures. Collagen in the treated group showed a greater extent of lysine hydroxylation, higher levels of hydroxylysine-aldehyde derived cross-links and accelerated cross-link maturation compared with the untreated group. Furthermore, the treated group showed accelerated matrix mineralization. These results indicate that FCP exerts a positive effect on osteoblastic cells in terms of collagen synthesis, quality and mineralization, thereby suggesting the potential utility of FCP for bone tissue engineering.

Information related to the author
© 2013 The Japanese Society for Dental Materials and Devices
Previous article Next article
feedback
Top