Dental Materials Journal
Online ISSN : 1881-1361
Print ISSN : 0287-4547
ISSN-L : 0287-4547
Original Paper
Chemical alteration by tooth bleaching of human salivary proteins that infiltrated subsurface enamel lesions —Experimental study with bovine lesion model systems—
Junko IIZUKAYoshiharu MUKAIMotoe TANIGUCHIYuko MIKUNI-TAKAGAKIJacob Martien TEN CATEToshio TERANAKA
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2014 Volume 33 Issue 5 Pages 663-668

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Abstract
Salivary macromolecules infiltrate white and brown spot enamel lesions and adsorb onto hydroxyapatite. Calcium-binding salivary proteins such as statherin hinder remineralization of these lesions. We assessed whether bleaching agents can remove salivary components that have infiltrated and bound to experimental subsurface lesions in bovine enamel prepared by immersing specimens in acid and then human saliva. Transversal microradiography showed that such demineralized lesions mimicked incipient carious lesions. Bound proteins to the experimental and untreated control specimens were eluted in a stepwise manner with phosphatebuffered saline, 0.4 M phosphate buffer, and 1 M HCl. SDS-PAGE of dialyzed extracts showed that specific salivary proteins bound to the lesions, while virtually no protein bands were detected if the specimens were bleached. Western blotting showed that even statherin, which was more firmly bound than other proteins, was removed. In-office bleaching agent may be useful in treating enamel lesions for removing proteins bound to these lesions.
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© 2014 The Japanese Society for Dental Materials and Devices
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