Dental Materials Journal
Online ISSN : 1881-1361
Print ISSN : 0287-4547
ISSN-L : 0287-4547
Original Paper
Cytotoxicity of commonly used luting cements —An in vitro study
Rita TRUMPAITE-VANAGIENEVirginija BUKELSKIENEJolanta ALEKSEJUNIENEAlina PURIENEDaiva BALTRIUKIENEVygandas RUTKUNAS
Author information
JOURNAL FREE ACCESS

2015 Volume 34 Issue 3 Pages 294-301

Details
Abstract
The study aimed to 1) evaluate the cytotoxicity of luting cements: Hoffmann’s Zinc Phosphate (Hoffmann’s ZP), GC Fuji Plus Resin Modified Glass Ionomer (Fuji Plus RMGI) and 3M ESPE RelyX Unicem Resin Cement (RelyX Unicem RC) and 2) test if pre-washing reduces the cements’ cytotoxicity. In vitro human gingival fibroblast (HGF) culture model was chosen. The cytotoxicity was evaluated by MTT test, the cell viability —by staining the cells with AO/EB dye mixture. The means±SD of Cell Survival Ratio (CSR%) were compared among different cement types under two testing conditions, with or without cement pre-washing. The CSR%s were compared by ANOVA and linear multiple regression (LMR). Hoffmann’s ZPC was less cytotoxic, while Fuji Plus RMGIC and RelyX Unicem RC were more cytotoxic (ANOVA, p<0.001). The type of cement and cement pre-washing jointly explained 90% of cell survival (LMR, p<0.001, adjusted squared R=0.889). The commonly used luting cements such as Hoffmann’s ZP, Fuji Plus RMGI and RelyX Unicem RC may have a cytotoxic potential.
Content from these authors
© 2015 The Japanese Society for Dental Materials and Devices
Previous article Next article
feedback
Top