Dental Materials Journal
Online ISSN : 1881-1361
Print ISSN : 0287-4547
ISSN-L : 0287-4547
Original Paper
Fibronectin-coated microgroove titanium surface promotes the behaviors of human gingival fibroblasts
Sijia HUANGYanjun LINLin ZHOU
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2023 Volume 42 Issue 4 Pages 509-515

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Abstract

The present study was designed to clarify the activity of human gingival fibroblasts (HGFs) on the fibronectin (FN)-coated silanized microgroove titanium surface. The surface elemental composition of titanium discs was detected using XPS. HGFs’ adhesion to the titanium discs was detected by immunofluorescence staining of vinculin. HGFs’ number on the titanium discs was detected using the CCK8 assay. HGFs’ secretion of type 1 collagen after five days of culturing was detected using ELISA and qPCR. HGFs could proliferate and spread well on the surface. The viability of HGFs in the experimental group was significantly more than in the control group. The HGFs in the experimental group significantly secreted more type 1 collagen than in the control group. Therefore, FN-coated can improve the morphology, viability, and type 1 collagen secretion of HGFs silanized microgroove titanium surface, which might ameliorate the efficacy of implants.

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© 2023 The Japanese Society for Dental Materials and Devices
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