Article ID: 2019-209
We investigated the adsorption of lipoteichoic acid (LTA) on titanium surfaces by using the quartz crystal microbalance (QCM) method, and confirmed the influence of mucin (MUC) pre-adsorption on LTA adsorption. Two injection methods were evaluated. Namely, Method A: single-step injection of LTA solution to the Ti sensor, and Method B: MUC solution was injected to the Ti sensor followed by LTA injection on the MUC pre-adsorbed surface. QCM measurement revealed that the adsorbed amount of LTA by Method A was low and constant regardless of the increase in LTA concentration. In contrast, the adsorption amount of LTA in Method B increased according with increasing concentration of LTA and was significantly higher than that of Method A. The hydrophobic surface after MUC pre-adsorption was presumed to contribute to the enhancement of LTA adsorption. Our results revealed that MUC pre-adsorption to Ti is necessary for LTA adsorption in living tissue.