Dental Materials Journal
Online ISSN : 1881-1361
Print ISSN : 0287-4547
ISSN-L : 0287-4547

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Surface functionalization of titanium substrates with Deoxyribonuclease I inhibit peri-implant bacterial infection
Can SHAOXin ZHANGJing YEYa-Chong LIYi-Jun BAOZhi-hui LIYing HUANGYing LIU
著者情報
ジャーナル フリー 早期公開

論文ID: 2020-055

この記事には本公開記事があります。
詳細
抄録

This study aimed to investigate the effect of Deoxyribonuclease I (DNase I) coating on initial adhesion and biofilm formation of peri-implant bacteria. Titanium (Ti), Ti-polydopamine (Ti-PDOP), Ti-PDOP-DNase I and Ti-PDOP-inactivated DNase I samples were studied. The FE-SEM, EDS and XPS were used to confirm that DNase I was coated onto Ti. The initial adhesion and biofilm formation of Aggregatibacter actinomycetemcomitans (A.a) and Fusobacterium nucleatum (F.n) were observed by CLSM. The osteogenic induction of Ti-PDOP-DNase I on MC3T3-E1 cells was investigated by ALP activity and RT-PCR. The adhesion clearance rate of viable bacteria on the surfaces of Ti-PDOP-DNase I was 91.95% for A.a, and 96.37% for F.n, and the 24 h biofilm formation of the bacteria was significantly inhibited. In addition, on DNase I coating, the mRNA level of osteogenic marker genes (alp, opn, bsp, sp7) and the activity of ALP were both up-regulated. Therefore, DNase I coating could be an alternative approach for preventing implant-related infection.

著者関連情報
© 2020 The Japanese Society for Dental Materials and Devices
feedback
Top