Experimental study of the biological properties of nmHA-SiO₂ fiber materials prepared by electrospinning technology

Abstract

To study the biocompatibility of nanohydroxyapatite (nmHA)-SiO₂ fiber material and its efficacy in guided bone regeneration. ① The cytotoxicity of the nmHA-SiO₂ fiber material to MC3T3-E1 cells was determined by CCK-8 assay. The adhesion of cells on the surface of the material was observed. ② Bone defects were prepared in the skull of three groups of New Zealand white rabbits. The following treatments were administered: implantation of nmHA-SiO₂, implantation of Bio-Oss, and no treatment. The defects were then covered with nmHA-SiO₂ membrane or Hai’ao oral repair membrane. Animal samples were analyzed by gross observation, micro-computed tomography, hematoxylin-eosin staining and Masson staining. The data were statistically analyzed by multivariate analysis of variance to evaluate the repair of bone defects. ① The nmHA-SiO₂ fiber material has suitable biocompatibility. ② The nmHA-SiO₂ fiber material performed more effectively as a barrier membrane than other bone substitute materials in GBR model rabbits.