Cytotoxicity of a Trial Resin Composite Liner Containing TiK₂F₆ on Rat Dental Pulp Cells

Abstract

The aim of this study was to assess the toxicological responses of a resin composite containing TiK₂F₆ and NaF in rat dental pulp cells. Trial resin composite liners were made, containing 3wt% fluorides (TiK₂F₆ or NaF). These specimens were immersed in 5ml of cell culture medium supplemented at 37°C for 24 hours. The eluates were used for the experiments. We judged the cytotoxicity of the samples by the cell viability. The original elute solution was serially diluted and then the medium was exchanged for the dilute medium. The cell viability at 1, 2 or 5 days after commencement of re-culturing was calculated. The viability of cells in the eluate from the resin composite liners containing TiK₂F₆ and NaF decreased with time. The cytotoxicity of TiK₂F₆ was weaker than that of NaF at all times.