Abstract
We examined the bactericidal and virucidal effectiveness of a denture cleaner that uses ozone (ozone concentration, 10ppm) against methicillin-resistant Staphylococcus aureus (MRSA) and T1 phage, respectively- In the bactericidal activity test, with the ozone supply turned on, the number of bacteria was 3.1×103CFU/mL at the beginning of the experiment, fell to 1.0×100 CFU/mL 10min later, and was 1.0×100CFU/mL or less afterwards. In contrast, when the ozone supply was cut off (air bubble only), the number of bacteria was 3.4×103CFU/mL at the beginning of the experiment, and had fallen to 3.0×103CFU/mL 60min later (no statistically significant difference). In the virucidal activity test, the number of phages was 1.2×106PFU/mL before ozone treatment, fell to about 1/10 of that number 10min later, and was 6.1×100 PFU/mL 40min later.
These results indicate that the use of ozone in this denture cleaner is effective against MRSA and viruses.
