New Surface Modification of Titanium Implant with Phospho-amino Acid

Abstract

The purpose of this study was to investigate a new biochemical surface modification technique for titanium implants using phospho-amino acid. Pure titanium disks were pretreated with 10N HCl and ultrapure water at room temperature for 30 minutes respectively. Then these disks were modified with either L-threonine (Thr) or O-phospho-L-threonine (P-Thr) at 37°C for 12 hours. X-ray photoelectron spectroscopy (XPS) chemically analyzed the modified surfaces. It was revealed that the N is peak which originated from Thr was not detected in the wide-scan spectrum of Thr-modified surface, whereas three peaks of N 1s, P 2s, and P 2p which originated from P-Thr were detected in the wide-scan spectrum of P-Thr-modified surface. Moreover, the P 2p peak of P-Thr which reacted with the surface significantly shifted to a lower binding energy (p<0.05). Based on the results of this study, it was concluded that P-Thr chemically bonded to the titanium surface treated with HCI.