Original Paper
A Dynamic Model for Simulating Recombinant Protein Production in Transgenic Lettuce for Optimum Environmental Control
2009 年 47 巻 3 号 p. 157-165
詳細
2009 年 47 巻 3 号 p. 157-165
A dynamic model for simulating recombinant protein production in transgenic lettuce for optimum environmental control was developed. The model comprised three main parameters, which were leaf fresh weight, the number of cells, and amount of protein in each leaf. The differential equations of the model were integrated using the modeling software STELLATM. The model was calibrated based on the measured data of transgenic lettuce plants which were grown in a plant factory for 50 days from seeding. The plants contained an introduced Cauliflower mosaic virus-35S promoter fused onto the β-glucuronidase gene. Simulations were conducted to investigate the effect of plant growth control on the productivity of recombinant proteins which have various stabilities. The simulations indicated the importance of recombinant protein stability. If the parameters corresponding to the production and degradation of a recombinant protein can be identified, the simulation will be a strong tool for devising an environmental control strategy for highly efficient recombinant protein production systems.
