e-Journal of Surface Science and Nanotechnology
Online ISSN : 1348-0391
ISSN-L : 1348-0391
Regular Papers
Non-Invasive Detachment of Vorticella from Calcium Alginate Membrane
Moeto NagaiHiroshi AsaiHiroyuki Fujita
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2013 年 11 巻 p. 25-28

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Non-invasive detachment of Vorticella from a substrate is essential for fabricating a microsystem based on Vorticella. Here, we dry sodium alginate solution and obtain sodium alginate films with fewer uncrosslinked sol regions. Calcium ions cross-link the alginate polymers of the membrane and form a calcium alginate gel. We first culture V. convallaria on the gel membrane. By dissolving the membrane with a chelating agent, we detatch cells non-invasively from the substrate and obtain cells with intact stalks, which can then be placed in a microsystem without waiting for the stalks to grow. The cells can be patterned by lift-off of the calcium alginate membrane. The proposed method provides new processing capabilities and allows for the design and fabrication of a system based on an actuator of Vorticella. [DOI: 10.1380/ejssnt.2013.25]
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この記事はクリエイティブ・コモンズ [表示 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by/4.0/deed.ja
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