Nonspecific Detection of Proteins by the Use of Electrochemical SPR Sensor

抄録

An approach to detect urinary proteins has been examined. An electrochemical surface plasmon resonance (SPR) sensor was used to nonspecifically detect proteins such as albumin by observing the direct protein adsorption onto the sensor surface. The new method enabled us to measure the concentration of proteins by just contacting the sample solution with the sensor surface. The adsorption of human serum albumin (HSA) seemed to be regulated by applying potential on the Au electrode that is the sensing element of the sensor. The concentration of the protein was obtained from the change in the SPR signal caused by the protein adsorption. Furthermore, the potential application facilitated detergent to remove the adsorbed protein, which enabled us to reuse the sensor.