Biofuel cell is a next-generation energy device that generates the electricity from renewable fuels such as glucose using redox enzymes as electrode catalyses. We re-constructed in vitro pentose phosphate pathway for 24-e oxidation of glucose-6-phosphate (G6P) into CO2 and linked it to mediated bioelectrocatalytic NADPH oxidation system in order to improve the energy density of biofuel cells. The electrolysis efficiency reached to 40% in the G6P oxidation by adjusting the composition of the enzymes in the pathway.
