抄録
Incubations of rat ventral prostate with testosterone-14C and 5a-dihydrotestosterone (5α-androstane-17β-ol-3-one)-3H revealed the gradual increase in uptake of both steroids into the tissue. Ratios of 3H/14C of the incorporated steroids were similar during the incubation periods, and this indicated that incorporation of both the steroids proceeded in the same pattern. Radioactivity in purified 5α-dihydrotestosterone from the incubated whole tissues showed a gradual increase in both 14C and 3H, and 3H/'4C ratio was also almost constant throughout the incubation period. Therefore, rate of the conversion of testosterone to 5α-dihydrotestosterone seems to be constant during the incubation.
A considerable difference in 3H/14C ratios of ethyl acetate extracts was observed between the various subcellular components after 30 min incubation. 3H/14C ratio in 5α-dihydrotestosterone, however, showed a small difference in all subcellular components. Most of the radioactivity incorporated into purified nuclei was attached to 5adihydrotestosterone.
