抄録
1) The influence of mesoxalate and various hormones, when added into the medium or administered to the animal previously to the experiments, on the glucose balance of the medium and glycogen formation in liver slices underthe glycogenetic and glycogenolytic ionic environments of the incubating medium were investigated.
2) Under the glycogenolytic environment, the addition of mesoxalate or mesoxalate with insulin into the medium caused a slight decreased or no effect on the glycogen synthesis in normal and alloxan liver slices, while, under the glycogenetic environment the added mesoxalate showed an increased effect in normal animal, while decreased effect on the glycogen synthesis in alloxan.
3) Daily administration of mesoxalate previous to the experiment, resulted in an inhibition against glycogenolysis-somewhat for the normal and more for the alloxan animal. A marked decrease in the glucose appearance into the medium was found under the glycogenolytic condition and under the glycogenetic condition there were marked increased glycogenesis in normal liver slices.
4) Under the glycogenolytic ionic composition, the addition of insulin or adrenalin into the medium, which was shown to be inhibitory on the glycogen formation, caused no definite effect in the case of adrenalin pretreatment, and a depressive effect in insulin pretreatment on the glycogen formation, whilst under the condition of glycogenesis large amounts of glycogen were produced in the slices with insulin or adrenalin previously injected.
5) No change or certain glycogenolysis took place by the addition of DOCA or ACTH into the medium, and glycogenolysis was inhibited by cortisone. In the experiment of the pretreatment with cortisone under the glycogenolytic or even under the glycogenesic environment a marked glycogenolysis was seen in both the normal and alloxan liver slices. With ACTH pretreatment a marked promoting effect on the formation of glycogen was observed under the glycogenetic condition in alloxan and somewhat less in normal liver slices were seen.
6) It is interesting to compare these results on the liver slices with those on the diaphragm; two particular points were observed, firstly the results on glycogen synthesis in the liver slices and diaphragm of the ACTH pretreated animal were quite similar to the result on the one of the adrenalin pretreated: secondly, in the animals pretreated with cortisone a definite discrepancy on the glycogen formation was observed between the liver slices and diaphragm.
