Biological Activities of Synthesized 20K and 22K h GH in Nb₂ Bioassay and IM-9 Radioreceptor Assay

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We investigated the bioactivities of the recombinant DNA-derived methionyl 20K h GH (20K-Met-h GH) and methionyl h GH (22K-Met-h GH). The growthpromoting activities in Nb2 cells of 20K-Met-h GH and 22K-Met-h GH were 10.7% and 93.5% of pituitary h GH (P-h GH), respectively. In the IM-9 lymphocyte assay, the binding activities of 20K-Met-h GH and 22K-Met-h GH h GH receptor were 29.0% and 87.1% of P-h GH, respectively. Our data demonstrate that 20K-Met-h GH may have weaker biological potency than P-h GH.