Vasoactive Intestinal Peptide Enhances Dopamine Accumulation in Primary Cell Culture of Rat Hypothalamus

抄録

The effect of vasoactive intestinal peptide (VIP) and PHI-27 on dopamine accumulation in cultured rat hypothalamic cells was investigated. VIP enhanced [³H] dopamine accumulation dose dependently. This effect was siffnificant at 10^-8-10^-5M VIP with a concomitant increase in intracellular cyclic AMP (cAMP), and reached its plateau level at 10^-6M VIP. VIP increased [³H] dopamine accumulation significantly within 15 min. PHI-27 and dibutyryl cAMP ((Bu)₂-cAMP) also enhanced [³H] dopamine accumulation. These results suggest that VIP enhances dopamine accumulation in hypothalamic cells by increasing intracellular cAMP.