抄録
Both the calcium activity and the leukocyte activity of parotin were relatively resistant to the action of chymotrypsin, and remained even after 7hrs. of digestion. The ultraviolet absorption of the perchloric acid-soluble fraction in digested parotin by chymotrypsin rapidly increased within 1hr. owever the shift of the isoelectric point of parotin as seen in the tryptic digestion was not seen by the chymotryptic digestion.
The following 3 fractions were separated from the parotin digested for 3 hrs.; the precipitate yielded at its isoelectric point, pH 5.4, an insoluble fraction in 80% concentration of acetone, and a soluble fraction in this acetone solution.Among these 3 fractions only the first, pH 5.4-precipitate, showed all biological activities.
