抄録
The hydrolyzates in the digested parotin by trypsin were separated into the 3 fractions by the isoelectric precipitation and acetone fractionation: the pH 4.5-precipitate, the insoluble fraction in the acetone of 80% concentration, and the soluble fraction in the same solvent. The first pH 4.5-precipitate was relatively pure in view of the paper electrophoresis and the paper partition chromatography, and showed the same biological activities as the parotin before digestion. The 80% acetone-insoluble fraction was the mixture of some hydrolyzates and showed only the leukocyte increasing activity. The 80% acetone-soluble fraction contained many hydrolyzates and was negative in the biological activities.
