Endocrine Journal
Online ISSN : 1348-4540
Print ISSN : 0918-8959
Stimulation by 1, 25-Dihydroxyvitamin D3 of Insulin Receptor Expression and Insulin Responsiveness for Glucose Transport in U-937 Human Promonocytic Cells
BEGOÑA MAESTROJAVIER CAMPIÓNNORMA DÁVILACONSUELO CALLE
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2000 Volume 47 Issue 4 Pages 383-391

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Abstract

In the present work, we demonstrate that treatment with 1, 25-dihydroxyvitamin D3 for 24 hours increased n a dose-dependent manner the levels of the two major insulin receptor (IR) mRNAs (11 and 8.5Kb) present in U-937 human promonocytic cells. These levels reached maximum values (1.8-fold 11Kb; 1.4-fold 8.5Kb) with the addition of 10-8M 1, 25-dihydroxyvitamin D3. In these optimal conditions the stimulatory effect of 1, 25-dihydroxyvitamin D3 was accompanied by increases in both IR capacity, and insulin responsiveness for glucose transport in these cells. Moreover, such increases appear to be mediated by an enhanced expression of the receptor for 1, 25-dihydroxyvitamin D3, measured at the level of both RNA and protein. These results provide evidence of 1, 25-dihydroxyvitamin D3 acting as genomic stimulator of the insulin response in the control of glucose transport.

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© The Japan Endocrine Society
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